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ASCP ASCP-MLT Exam Syllabus Topics:

ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. The area of the federal government that ensures that the laboratory is stocked with gloves for their employees when performing tests on patient blood sample is:

A) HIPAA.
B) OSHA
C) CDC.
D) FDA.

2. The correct set of patient data would be: Hgb A = 60%, Hgb S = 38%, Hgb A2 = 2%, Hgb F = 0% Patients with sickle cell trait commonly have Hgb A values between 40 and 60%, Hgb S values between 20 and 40% and Hgb A2 values between 2 and 3%.
A known sickle cell trait patient has a hemoglobin electrophoresis test performed. Which of the following hemoglobin percentage sets would most closely match this patient's diagnostic state?

A) Hgb A = 25%, Hgb S = 70%, Hgb A2 = 2%, Hgb F = 3%
B) Hgb A = 60%, Hgb S = 38%, Hgb A2 = 2%, Hgb F = 0%
C) Hgb A = 90%, Hgb S = 8%, Hgb A2 = 2%, Hgb F = 0%
D) Hgb A = 40%, Hgb S = 58%, Hgb A2 = 2%, Hgb F = 0%

3. PCT usually rises within 3-6 hours of infection. CRP also increases rapidly following infection, but is not as specific for infection as PCT. A rise in CRP could also occur with SIRS. Lactic acid is usually used to detect and monitor impaired circulation and tissue oxygenation in critically ill patients.
Chemistry
Of the three laboratory tests that are listed, which has proven to be most effective for early differentiation of systemic inflammatory response syndrome (SIRS) from sepsis due to its increase following infection and higher specificity?

A) C-reactive protein (CRP)
B) Lactic acid
C) Procalcitonin (PCT)

4. Black stopper tubes are used for glucose collections.
Question options:

A) False
B) True

5. Modified Thayer Martin agar at 35ºC with CO2 is the correct answer. This is a typical presentation of systemic gonorrhea and Neisseria gonorrhea grows best on MTM medium at 35ºC. under CO2. Systemic gonorrhea occurs in 1% of cases and results in purulent arthritis and rarely septicemia.
An 18-year-old woman woke up in the morning to find that her left knee was hot, swollen, and very painful.
She could not walk to work and a friend offered her a ride to the clinic. She had never had a swollen joint before. Physical exam revealed a tender knee joint which yielded purulent synovial fluid on aspiration.
Gram-negative intracellular diplococci were seen on Gram stain. She also had a cervical discharge that was cultured. The cause of her infection would grow best on:

A) LIM (Todd Hewitt Broth w/Colistin and Nalidixic Acid) broth with refrigerated temperature
B) Modified Thayer Martin agar at 35ºC with CO2
C) V (human blood) agar at 35ºC with CO2
D) Blood agar at 42ºC with microaerophiilic conditions

Solutions: